Thin-Layer Chromatography of Steroid Hormones Essay Example for Free

Thin-Layer Chromatography of Steroid Hormones Essay The purpose of this experiment was to test the solubility of various steroids in order to obtain more information about their structure. An unknown was also given to be included in the experiment. The test that was performed was thin-layer chromatography which required a glass plate. The known steroids used in this experiment were estradiol, hydrocortisone, cortisone, corticosterone and deoxycorticosterone. Introduction Steroid hormones are a subgroup of lipids. They are found in one of the primary endocrine glands in the body, the adrenal glands, which are located directly superior to the kidneys. The structures of the adrenal glands begin with the outer layer known as the cortex. This region is most commonly referred to as the adrenal cortex and provides the gland with approximately 80% of its total mass. Within the adrenal cortex there are three layers: the outermost layer is known as the zona glomerulosa, the middle layer is known as the zona fasciculata, and the innermost layer is known as the zona reticularis. Each of these layers is comprised of many cells that synthesize and secrete hormones appropriately named the adrenocorticoids. The second portion of the adrenal gland is known as the adrenal medulla. It is the inner region and account for the remaining 20% in each of the adrenal glands. The adrenal medulla is responsible for the release of epinephrine and norepinephrine into the bloodstream. Adrenocorticoids include three types of steroid hormones. Each class belongs to a specific layer in the adrenal cortex and each class secretes specific hormones. The first group of adrenocorticoids is referred to as mineralcorticoids. These hormones are secreted by the zona glomerulosa and are responsible for the regulation of both the reuptake of sodium and the secretion of potassium. The mineralcorticoid used in this experiment was the more potent, deoxycorticosterone. Abnormal levels of minercorticoids can be associated with hypertension and can be induced by various problems, such as, low blood sodium, high blood potassium, cardiac failure, kidney failure, or even cirrhosis of the liver. The second group of adrenocorticoids is referred to as the glucocorticoids. These are produced by the second layer in the adrenal cortex, the zona fasciculata. The functions of glucocorticoids include stimulating gluconeogenesis through the use of amino acids and breaking down of muscle proteins. In this experiment the glucocorticoids used were hydrocortisone and corticosterone. These hormones are used medically to suppress the immune system by inhibiting the inflammation process. However, too much secretion of these hormones can be indicative of Cushing’s syndrome, pregnancy, and stress that comes from disease or other sorts of bodily trauma. The third group of adrenocorticoids that will be discussed are sex hormones. Sex hormones are not only secreted by the adrenal cortex. They are largely secreted in the reproductive system of each respective gender. Sex hormones can be further categorized into androgenic hormones, estrogenic hormones, and progestational hormones. Androgenic hormones are found predominately in the testes of the male, and there is it secreted by the interstitial Leydig cells. The most common and most powerful androgenic hormone is testosterone. Estrogenic hormones are only slightly different from their counterparts. Estrogenic hormones are identified by their 18 carbon ring structure whereas androgens are identified by their 19 carbon ring structure. Estrogenic hormones are secreted by the female reproductive organs known as the ovaries. The most potent estrogenic hormone is called estradiol and its release fluctuates throughout a woman’s menstrual cycle, with its peak volumes being around the time of ovulation. The last subgroup of sex hormones is called progestational hormones, or progesterone. Progesterone is responsible for the final maturation of the endometrium. Located in the ovaries, it is released from the corpus luteum during the luteal phase of the menstrual cycle and also increases in volume during pregnancy as the fetus progresses. Progesterone is different from the other two classes of sex hormones in that it is a 21 carbon ring structure. The modality used in this experiment was a chromatogram. Thin-layer chromatography is the type of chromatography that uses a small glass sheet covered in a thin layer of an adsorbent material. Once solutes are obtained, they are carefully placed on the glass sheet on the origin line. Then the glass sheet is soaked in a solvent for a certain period of time that varies with each chromatography technique. After it is through soaking, the solutes can be observed and the distance that each solute has moved can be measured. This value is obtained by using the R? ormula which takes the distance a solute travels from the origin line divided by the distance that the solvent traveled (this measure is known as the solvent front). This R? value can help determine unknowns as well. The final result of this experiment is known as a chromatogram, and depending on which technique is used, the steroids can be seen through regular light, or via a UV lamp. A UV light lamp was required for this experiment. Chromatography is a very useful tool in identifying the solubility properties of steroid hormones and revealing variants in hormone structure.

The labour cost advantage and china

The labour cost advantage and china Introduction China has an emerging economy and therefore, produces many products. On the European market, there is a large demand for these products. China has relatively low production costs, which makes China an interesting country to import from. Chinese wages have risen sharply with globalization, average wages in China have increased every year since 1978. The average wage in urban areas was 21.000 Yuan in 2006, which is four times higher than the average wage in 1995. How will the wages develop in China? The question for this research is: Has China the labour cost advantage? Content Has China the labour cost advantage? 1 Introduction 2 Content 2 Offshoring in general 3 Why are companies outsourcing to China? 3 Employment in China 4 What was Chinas economic reason to enter the WTO? 4 How have wages been developed in China? 6 Wages by Ownership 7 Wages by Region 9 Wages by Sector 10 Wages compared with other countries in Asia 11 Has China the labour cost advantage? 12 Bibliography 13 Offshoring in general Offshoring is defined as the movement of a business process done at a company in one country to the same, or another company, in another country. Most of the movements to lower-costs destinations take place in the own management. Outsourcing is the movement of an internal business process to an external company in the same country and the movement of an internal business process to an external company in another country. Offshore outsourcing occurs most often.  [1]   Why are companies outsourcing to China? There are different reasons why companies outsource to China, but some of these reasons are similar. Several reasons for outsourcing to China are  [2]  : Cheap Labour: China offers savings up to five times compared to the U.S. The labour costs in China are 50 percent lower than in India. Labialization of laws and government policies: Government has passed laws which protect private ownership and intellectual property in the country. It has also embraced public/private partnerships and domestic/foreign partnerships. Existence of offshore manufacturing and physical proximity to major markets: China is the manufacturing plant of the world, which provides sound base for outsourcing, strengthened by risk mitigation and greater stability. Employment in China Although China is still a developing country with a relatively low average income, it has a tremendous economic growth since the seventies (9.1 percent in 2004). This can be related to a great extent of an economic liberalising policy. The Gross National Product raised with 400 percent between 1978 and 1998 and the international investments grew tremendous during the nineties. The agriculture is by far the most important sector. Figures of 2007 show that 41 percent of the total population of China is employed in this sector. Nevertheless, the agricultural land limits to around 11 percent of the total Chinese land surface. Since the seventies, the agriculture is privatised, which yield a tremendous production growth. Figure 1: Economic growth, percentage per year  [1]  4 What was Chinas economic reason to enter the WTO? Joining the WTO is a very important event for the development of China at the beginning of the 21st century. WTO membership opens up Chinas market for more international trade and investment, and opens up the world economy for Chinas exports. Some researchers see it as a positive force for Chinas economic development while others are concerned that the competition of foreign imports and foreign enterprises in China might destroy important domestic enterprises in Chinas agricultural, manufacturing and service sectors. The main motivation of Premier Zhu Rongji in promoting Chinas entry into the WTO was to use foreign competition to speed up economic reform in both the industrial and service sectors. In the late 1990s, reform in both sectors was slow due to the inertia coming from vested interests of a group of formerly appointed managers holding on their positions.  [3]   How have wages been developed in China? Average wages have increased every year since 1978. In 2006, the average wage in urban areas in was 21.000 Yuan, which is four times higher than the average wage in 1995. However, as wage levels increased, so did discrepancies between different sectors, types of ownership and regions. In general, average wages were higher in share-holding, foreign-owned and state-owned enterprises, and were lowest in locally funded enterprises, with wages in enterprises owned by Hong Kong and Taiwanese businesses in the middle. A more significant gap emerged between different occupations and industrial sectors, and especially between low-skilled and high-skilled workers.   In 2006, the average wage of employees in primary industries was only 786 Yuan, which is a quarter of the average wage of employees working in financial services (3.273 Yuan) and one-fifth of those working in the computer industry (3.730 Yuan). Wages by Ownership Between 1995 and 2007, the average annual wage for employees grew more than four times, from 5.600 Yuan to 22.700 Yuan. Figure 2 shows the average annual wages of staff and workers by type of ownership from 1995 until 2007. In China, there were three periods of wage reform. The first period of reform started around 1985. Before this year the average wage growth was around 4.9 percent per year. In the period 1986 until 1997, employment in jointly owned enterprises experienced a tremendous growth. The average wage growth per year was still quite low, with an average of 3.9 percent, which was partly due to a negative growth in 1988 and 1989 (because of inflation and political upheaval). The third period was from 1997 until 2007. From 1999 on, the average wages were rising rapidly with an average of 14 percent per year. This could be because of Chinas preparation for getting into the WTO, as well as the restructuring of state-owned enterprises which started in 1998. Wages in the state sector began to increase in the late 1990s, reaching 14.358 Yuan in 2003, surpassing private sector wages by a narrow margin for the first time since reform began. By 2007, the average wage in the state sector was about 11 percent higher than in the private sector. The state-owned sector has been restructured in the 1990s. In the planned economy, they had low productivity, disguised unemployment (because of Chinas political function of maintaining low unemployment) and limited profits. They had a wage system which was dependent on seniority. In the 1990s, the Chinese government would not include the losses of their enterprises. This is why they began with restructuring. They started by allowing privatisation of small and medium state-owned companies. After that, the government started with a more aggressive restructuring. The objective was to shut down loss-making companies and establish modern forms of corporate governance. These reforms led to many layoffs in state-owned companies. From 1996 to 2002, around 40 million employees were laid off. As can be seen in figure 3, the Chinese government succeeded in downsizing the employees and the productivity in the state-owned sector increased. Figure 2: Annual wages of staff and workers by type of ownership in thousand Yuan Source: China Statistical Yearbook Figure 3: Employment share and labour productivity for state owned enterprises Source: http://www.conference-board.org/economics/workingpapers.cfm?pdf=E-0024-07-WP Wages by Region Figure 4 shows the annual wage of employees divided in different regions in China. The 30 provinces are divided by the National Bureau of Statistics of China in six regions: Bohai (Beijing and surrounding provinces), Southeast (including Shanghai, Guangdong and other coastal provinces), Northeast, Central, Southwest and Northwest China. Tibet is not listed, since there is limited information about this region. Figure 3 presents the real annual wage of employees across the six regions in China, showing the different wage patterns. During the first period of reform, the average wages were grouped. Later on, the wages in the South-eastern and Bohai regions began to rise. As can be seen in figure 4, the wages in the South-eastern and the Bohai regions now have the highest average wages. The difference between these two regions and the other four regions is around 30 to 40 percent. The highest growth in the past 20 years has occurred in the South-eastern and the Bohai regions, the coastal areas where cities as Beijing, Guangzhou, Shanghai and Shenzhen are located. Figure 4: Annual wages by Region in thousand Yuan (Source: China Statistical Yearbook) Wages by Sector The wages by sector have about the same pattern as the wages by regions. In figure 5 the wages across sectors are showed. Remarkable is that the wages across sectors stayed clustered until 1993. After 1993, the average wages for Banking Insurance, Scientific research Polytechnic service increased rapidly. These two sectors are both sectors with skill intensive labour. To make a comparison between the skill intensive sectors and the other sectors (unskilled sectors), the average wage in the construction sector was 5.729 Yuan in 1990, about 14 percent higher than the average wage for the banking Insurance sector. In 2007, the Banking Insurance sector had an average wage of 50.000 Yuan, around 265 percent higher than the wages in the Construction sector. People believed that manufacturing wages grew the fastest because of the increasing volume of exports in China. Actually, these wages grew even below the national average, although manufactured products counted for more than 90 percent of Chinas export. Figure 5: Annual wages by sector in thousand Yuan (Source: China Statistical Yearbook) Wages compared with other countries in Asia Figure 6 shows the manufacturing wages of different Asian countries from 1979 to 2007. The wages of China are comparable with the Philippines and Thailand, plausible is that the wages of Malaysia are much higher than the other countries listed. Figure 6: Annual Manufacturing wages between several Asian countries in USD Source: International Labour Organization Has China the labour cost advantage? Wages have been developed. However, there are differences between skilled and unskilled labour. The wages for skilled labour increased rapidly the past decade. (see page 10)Wages for unskilled labour still remains quite low compared to the skilled intensive sectors. Reasons for the wage growth of unskilled workers are the GDP growth of more than 10 percent per year, and the export growth of almost 30 percent per year since China became part of the WTO in 2001. In contrast to this, employment in manufacturing has increased with 3 percent in recent years. Compared to other developing countries in Asia, China still have the lowest wages on average. However, wages in China have developed rapidly since the late 1990s. Another factor is the large supply of graduated students over the past several years. Due to an increasing demand in skilled labour, the Chinese government expanded the enrolment of students in the late 1990s. In 1998, the total number of admitted students was around 1.08 million, a number that increased to 2.68 million in 2001. This could prevent a rapid increase of the wages in the future.

Lewis Carrolls Through the Looking Glass Essay -- Literature Children

Lewis Carroll's Through the Looking Glass â€Å"If it was so, it might be; and if it were so, it would be; but as it isn’t, it ain’t. That’s logic,† according to Tweedledee, a character in Lewis Carroll’s famous children’s work Through the Looking Glass (Complete Works 181). Of course, Lewis Carroll is most well known for that particular book, and maybe even more so for the first Alice book, Alice’s Adventures in Wonderland. The connection between Lewis Carroll and logic is less obvious for most people. In reality, Lewis Carroll is the nom de guerre for the Reverend Charles L. Dodgson, a â€Å"puttering, fussy, fastidious, didactic bachelor, who was almost painfully humorless in his relations with the grown-up world around him† (Woollcott 5). Though it may seem that Dodgson and his pseudonym emit two very different personalities, as Braithwaite points out, there really only existed â€Å"a completely integrated though singular personality† (174). While Dodgson under his true name usually only published books on mathematics and logic, under the name of Lewis Carroll he published books for the young, with some exceptions. One such exception to this division of subjects is the work Symbolic Logic; this textbook was published under the name of Lewis Carroll. It is through Dodgson’s children’s works that his integrated personality emerges. His Alice books, for example, contain many statements of logic and games of mathematics, intended for the amusement of his audience. Dodgson â€Å"regarded formal and symbolic logic not as a corpus of systematic knowledge about valid thought nor yet as an art for teaching a person to think correctly, but as a game† (174). With this perspective, it is easy to see why he was interested in... ...tin. The Universe In A Handkerchief. New York: Copernicus, 1996. Gardner, Martin. The Annotated Alice. New York: W. W. Norton & Company, 2000. Gattegno, Jean. Lewis Carroll: Fragments of a Looking-Glass. New York: Thomas Y. Crowell Company, 1974. Goldfarb, Nancy. â€Å"Carroll’s Jabberwocky.† The Explicator 57 (1999): 86. Hofstadter, Douglas R. Gà ¶del, Escher, Bach: An Eternal Golden Braid. New York: Basic Books, 1979. Holmes, Roger W. â€Å"The Philosopher’s Alice in Wonderland.† Phillips 159-174. Phillips, Robert, ed. Aspects of Alice: Lewis Carroll’s Dreamchild as seen through the Critics’ Looking-Glasses. New York: Vanguard Press, 1971. Wilson, Edmund. â€Å"C. L. Dodgson: The Poet Logician.† Phillips 198-206. Woollcott, Alexander. Introduction. The Complete Works of Lewis Carroll. By Lewis Carroll. New York: Random House. 1-9.

Sassouma Berete and Sogolon Kedjou in The Epic of Sundiata Essay

Mothers play a very influential role in a young mans life. Even though Sogolon Kedjou and Sassouma Bà ©rà ©tà © both had an impact on there son's life, their character traits were at different ends of the maternal spectrum. Despite minuscule similarities Sogolon Kedjou and Sassouma Bà ©rà ©tà © were depicted in direct contrast in the epic Sundiata. When comparing the two Sogolon Kedjou and Sassouma Berete both did what they thought would help to get their son's to become king. Sogolon knew everything that Sassouma was capable of doing to hurt her family, so she came up with the idea to leave the kingdom. Soglon said, "Lets us leave my son" (113). She thought this would be the best thing to do for all her children so she could keep them alive. "Despairing of ever injuring you, Sassouma will aim her blows at your brother or sister"(115). Although it was Sundiata's destiny to become king there was no set time placed on when this would occur. Therefore traveling to different kingdoms gave them time, and allowed for the fulfillment of not only Sundiata's destiny, but the destiny of his sibling too. Sassouma on the other hand wanted her son Dankaran to reign as the king of Mali after his father passed away. Sassouma knew her late husband would not pass the throne to her son, so she decided to banish Sogolon and her family to the backyard. Sassouma thought this would help her son's path to becoming the next king of Mali. She thought Sundiata was competition and a distraction to her son, so she wanted him dead. "I want to kill Sundiata. His destiny runs counter to my son's and he must be killed while there is still time" (115). Although Sogolon and Sassouma Berete were both wives and mothers, Sogolon was portrayed as a good mother... ... to mine" (111). From this quote, it clearly shows how much arrogance and vanity the queen really had. In contrast to this, and despite the intense humiliation that she went through, Sogolon humbly left Sassouma's home "with a lump in her throat."(111) She never talked back to Sassouma, and she would never let Sassouma see her cry. Since Sogolon and Sundiata let his fait play out, they ended up on top. Sundiata takes his rightful place and becomes the king of Mail which he was born to lead. In conclusion, despite minuscule similarities Sogolon Kendjou and Sassouma Berete were depicted in direct contrast in the epic Sundiata. It is obvious that, despite the fact that both queens wanted the best for their sons, their characters and methods were different. Since they were so different it influenced not only Sundiata and Dankaran but all of the king's children.

The Causes Of World War I :: essays research papers

The first world war had many causes, the historians probably have not yet discovered and discussed all of them so there might be more causes than what we know now. In this essay I would discuss the main causes of the war—the ones that were discussed in the textbook. In the time before the war’s outbreak (before 1914—in the beginning of the century) the world was separated into big empires that had colonies around the world (European countries ruled most of Africa). There were fights and disagreements between the major empires. Usually, when there are many arguments between countries, people start fear of war and prepare for it (for example, today in Israel, after Benjamin Netanyahu winning the elections, Israel’s policy towards the Arabs and the Palestinians have changed. There is fear from war with Syria, because of threats from both sides, so both countries arm themselves and ready for war, just in case it would break). In the beginning of the century Britain was the largest empire in the world, it also had the largest navy. The navy was so big and strong because the Britons needed to protect their empire and maintain the sea routes between the different colonies. The Kaiser William II of Germany hated and envied Britain for having a stronger navy than his. He increased the German navy and built many warships. Britain responded with building more ships and increasing its navy too. This started a race for building more and better warships and it created tension and competition between those two countries. Germany and France were rivals for centuries and at the beginning of the century and at the end of the 19th century the rivalry increased. This happened because of the war between those two countries in 1870-71 when Germany defeated France. Germany took over the areas of Alsace and Lorraine and the French people wanted to revenge Germany and retrieve those lands. France could not start a war against Germany since Germany had more resources and a stronger army than France. France needed allies to help it fight (the subject of alliances would be discussed later). There was another quarrel between France and Germany—about controlling Morocco. In 1905 France thought that it should have more influence in the western Mediterranean Sea area as it already controlled Tunisia and Algeria, it wanted Morocco too. The Kaiser of Germany visited Tangier (a big city in Morocco) and said that Germany would protect Morocco in case of a French attack. In 1911 France increased its control over Morocco and Germany sent a warship. The Germans withdrew at last but the French gave them a part of Western Africa in exchange.

Eastern Gear

1. What are the major problems being faced by Eastern Gear? The major problem’s Eastern Gear is facing is that they are accepting large orders to help pay their overhead, also, their sales group is not part of the business, there is no link between them and manufacturing. Their order entry is inefficient; the tolerance on products is not firm. The layout of their shop is set up to make mistakes and increased lead time has resulted in the need for an expeditor. Lastly, they are hiring too many employees for the company’s needs. 2. What action should Rhodes take to solve his problems? First, Rhodes needs to stop accepting large orders at discounted prices to help cover overhead, doing this will not cover overhead and should only be done if the company is facing bankruptcy. Accepting the large orders causes some of the small orders to wait for processing and in-turn, are late. Also, the sales group is not part of the business, there is no direct link between them and manufacturing. Therefore, no one is paying attention to the order size. This needs to be corrected by putting a direct line of communication between sales and manufacturing. Second, the order entry system needs to be more efficient. A customer is able to request a change in design after the order has already been placed, it may be necessary to stop production on these orders and wait for the new raw materials or for the new design to be clarified. They do not have a process that charges back the variance on these orders; they need to input a system where if the customer wants a design change, they have to pay for it. Also, the customer’s prints submitted with the order do not always contain the tolerances required during machining. This is a discipline issue within the organization that needs to be eliminated; the customer must sign off on the tolerances before the order begins processing. Third, Eastern Gear needs to change the layout of the shop. Lead time has recently increased from 2 to 4 weeks, which made it necessary to hire an expeditor, there is a concerning amount of bottlenecks in the production process and inspection of the products is not done until the order is completed. The shop is set up to make production errors and ship unfinished product. The tools need to be central and equipment should not be set up by type but placed in proper sequence so that the product moves sequentially from the beginning of production to the end, and quality checks and inspection should be incorporated in the production process. Also, Eastern Gear needs to change their order fulfilment approach from made-to-order to made-to-stock. Changing the layout of the shop and order fulfilment approach will help reduce bottlenecks and lead time as well as eliminate the need for an expeditor. Lastly, Eastern Gear has a workforce of 50 people and is managed using a family- type approach. They have hired 10 new employees within the last quarter. The typical order spends 90% of the time waiting for a machine to become available, and only 10% of the time is actually spent processing the order, they should not hire more employees. 3. How can this case be related to operations strategy and process design concepts? This case related to operations strategy and process design concepts because having a good operations strategy and process design are key to running a successful manufacturing company. As witnessed in this case, the operations strategy and the process design need adjustments in order to be successful. Eastern Gear 1. What are the major problems being faced by Eastern Gear? The major problem’s Eastern Gear is facing is that they are accepting large orders to help pay their overhead, also, their sales group is not part of the business, there is no link between them and manufacturing. Their order entry is inefficient; the tolerance on products is not firm. The layout of their shop is set up to make mistakes and increased lead time has resulted in the need for an expeditor. Lastly, they are hiring too many employees for the company’s needs. 2. What action should Rhodes take to solve his problems? First, Rhodes needs to stop accepting large orders at discounted prices to help cover overhead, doing this will not cover overhead and should only be done if the company is facing bankruptcy. Accepting the large orders causes some of the small orders to wait for processing and in-turn, are late. Also, the sales group is not part of the business, there is no direct link between them and manufacturing. Therefore, no one is paying attention to the order size. This needs to be corrected by putting a direct line of communication between sales and manufacturing. Second, the order entry system needs to be more efficient. A customer is able to request a change in design after the order has already been placed, it may be necessary to stop production on these orders and wait for the new raw materials or for the new design to be clarified. They do not have a process that charges back the variance on these orders; they need to input a system where if the customer wants a design change, they have to pay for it. Also, the customer’s prints submitted with the order do not always contain the tolerances required during machining. This is a discipline issue within the organization that needs to be eliminated; the customer must sign off on the tolerances before the order begins processing. Third, Eastern Gear needs to change the layout of the shop. Lead time has recently increased from 2 to 4 weeks, which made it necessary to hire an expeditor, there is a concerning amount of bottlenecks in the production process and inspection of the products is not done until the order is completed. The shop is set up to make production errors and ship unfinished product. The tools need to be central and equipment should not be set up by type but placed in proper sequence so that the product moves sequentially from the beginning of production to the end, and quality checks and inspection should be incorporated in the production process. Also, Eastern Gear needs to change their order fulfilment approach from made-to-order to made-to-stock. Changing the layout of the shop and order fulfilment approach will help reduce bottlenecks and lead time as well as eliminate the need for an expeditor. Lastly, Eastern Gear has a workforce of 50 people and is managed using a family- type approach. They have hired 10 new employees within the last quarter. The typical order spends 90% of the time waiting for a machine to become available, and only 10% of the time is actually spent processing the order, they should not hire more employees. 3. How can this case be related to operations strategy and process design concepts? This case related to operations strategy and process design concepts because having a good operations strategy and process design are key to running a successful manufacturing company. As witnessed in this case, the operations strategy and the process design need adjustments in order to be successful.

Agreed Ways Of Working

‘Agreed ways of working’ is working with an individual with their preferences, wishes and needs in mind. You will find this out when you read the individuals care and support plans as well as communicating with them. It is important that you follow the individuals care and support plans and understand and respect what the individual says they need. You have a professional duty of care to the individuals you support. Your role is to guide and support them and to help them live as independently as possible. You should listen to individuals and never put pressure on them.The importance of having full and up-to-date details of the agreed ways of working.  It is extremely important that the individual feels in control of any decisions that is made on their behalf. The individual must feel they can trust you to act on their behalf without agreeing to something the individual disagrees with. If the individual does not feel they can trust you, this can cause them unnecessary s tress and lead them to do things they do not want to do. You must ensure you are working and making decisions with the individuals beliefs, views and opinions in mind.Why it is important that you follow the limits of your job roleIt is important that you follow the guidance about the limits of your job role so that you are able to know what duties you are supposed to perform. Going beyond these limits could result in situations you are not equipped for and may affect the health and safety of yourself, other staff or service users.IF YOU ARE UNSURE OF ANYTHING OR HAVE ANY QUESTIONS, JUST COME AND ASK.